Leviatan S, Vogl T, Klompus S, Kalka IN, Weinberger A, Segal E. *Immunity* · December 13, 2022 · DOI: [10.1016/j.immuni.2022.11.004](https://doi.org/10.1016/j.immuni.2022.11.004) How to cite ### AMA Leviatan S, Vogl T, Klompus S, Kalka IN, Weinberger A, Segal E. Allergenic food protein consumption is associated with systemic IgG antibody responses in non-allergic individuals. *Immunity*. 2022;55(12):2454-2469.e6. doi:10.1016/j.immuni.2022.11.004 ### APA ``` Leviatan, S., Vogl, T., Klompus, S., Kalka, I. N., Weinberger, A., & Segal, E. (2022). Allergenic food protein consumption is associated with systemic IgG antibody responses in non-allergic individuals. *Immunity*, 55(12), 2454–2469.e6. https://doi.org/10.1016/j.immuni.2022.11.004 ``` ### BibTeX ``` @article{leviatan2022allergenic, title = {Allergenic food protein consumption is associated with systemic IgG antibody responses in non-allergic individuals}, author = {Leviatan, Sigal and Vogl, Thomas and Klompus, Shelley and Kalka, Iris N. and Weinberger, Adina and Segal, Eran}, journal = {Immunity}, volume = {55}, number = {12}, pages = {2454--2469.e6}, year = {2022}, doi = {10.1016/j.immuni.2022.11.004} } ``` Leviatan and colleagues used PhIP-Seq to measure systemic IgG binding against tens of thousands of food and environmental allergen peptides in a population-scale Israeli cohort. They found that common food antigens elicit measurable systemic IgG in up to half of non-allergic adults — far above clinical allergy prevalence — and that those antibody repertoires correlate with dietary intake. In a five-year longitudinal subcohort, the IgG epitope pattern was highly correlated within individuals but not between random pairs, behaving as a durable molecular fingerprint of an individual's diet. [ Read publication at Immunity ](https://doi.org/10.1016/j.immuni.2022.11.004) In this publication: - 1,003 Israeli adults profiled across 58,233 phage-displayed allergen peptides via PhIP-Seq, totaling 58 million antibody–peptide interactions. - Common food antigens elicited systemic IgG in up to 50% of non-allergic adults — far above the <1% population prevalence of food allergy. - An average of 322 ± 106 peptides were significantly enriched per person; hierarchical clustering surfaced 13 antibody-binding clusters spanning food and environmental antigens. - In a 214-person longitudinal subcohort sampled five years apart, IgG epitope repertoires were highly correlated within individuals but not between random pairs — a durable serological fingerprint of dietary history. This study repositions food-directed IgG from a presumed pathology signal to a quiet, baseline immunological record of consumption. Using PhIP-Seq (phage immunoprecipitation sequencing), the authors profiled IgG binding against 58,233 peptides drawn from food and environmental allergen databases in 1,003 Israeli adults — 58 million antibody–peptide interactions in total — and detected, on average, 322 ± 106 peptides significantly enriched per person. Hierarchical clustering surfaced 13 antibody-binding clusters spanning food and environmental antigens, with extensive cross-reactivity across phylogenetically related cereals, milk proteins, poultry, and legumes. Parallel IgG- and IgA-specific capture experiments confirmed the binding signal is overwhelmingly IgG. In a 214-individual longitudinal subcohort sampled five years apart, food-directed IgG epitope repertoires were highly correlated within individuals but not between random pairs — functioning as a five-year-stable molecular fingerprint of dietary history. For allergists separating clinically meaningful IgE-mediated sensitization from background reactivity, the work quantifies the non-allergic IgG baseline that any food-allergy biomarker must be measured against. For nutrition and microbiome researchers, it offers a serological lens on the diet–immune axis that can be triangulated with diet diaries, anthropometrics, and microbiome composition in the same cohort. [← Back to all resources](/) ## Related resources [![](https://cdn.sanity.io/images/vbcbvda6/production/257430cd1699782d6d265a15eff4a7e3dbfa6411-1200x750.webp)Webinar ### Antibody Reactome Profiling for Human Health Why reactome profiling captures biology that other immune assays miss. 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